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T-2 is a mycotoxin commonly found in Fusarium-infected wheat. Due to its phytotoxic effects, an understanding of its impact on wheat seedling development, heading stage, yield, and quality is necessary. It was determined that T-2 reduced the percentages of emerged seedlings (PES) and emerged seedlings at BBCH 13 (PES25) and emerged seedlings at BBCH 20 (PES20). It delayed flowering and reduced seedling and grain yields of four wheat cultivars. Temporarily lower concentrations of T-2 reduced the percentages of emerging seedlings (PES50), emerged seedlings at BBCH 13 (PES25), emerged seedlings at BBCH 20 (PES20), and reduced the seedling weight (SW). However, high concentrations of T-2 increased PES50, PES25, PES20 and reduced SW. As a result, DW varied significantly in the 0-200 μg/g dw T-2 treatment. The numbers of spikes in T-2-treated plants were lower, and the average number of grains per spike was lower. Regarding T-2-induced changes in grain quality, we observed that T-2 increased the amylase activity and reduced the glutathione reductase activity, which may have led to reduced grain quality, reflected in the T-2-induced lower germination rates, lower numbers of grains per spike, and lower flour protein content in the T-2-treated plants compared with the control.The contribution of water to energy transport in the protein core of the ribosome: biological implications.
The ribosomal RNA (rRNA) core and associated ribosomal proteins (r-proteins) are considered to be large water-filled cavities in the ribosome. The proton uptake by the ribosome, as well as the proton-release channel activity, have been studied and compared with that of the protein core in a detergent-free (zwitterionic) buffer. The buffer protonation equilibriums were determined by the Stewart-Rubin method using acid-base titrations. Results show that the hydration of the RNA is weaker than that of the proteins. The average number of water molecules per phosphate group of RNA
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